Chapter 04 / Fragment visibility
Green-channel assay
Slide 18
Faint bands make the strongest signal.
Amplification confidence by visible lane intensity
n=4,812 gel-doc runs
Lane intensity score
calibrated to reference ladder
100
75
50
25
0
31%
trace
44%
low
58%
visible
73%
clear
86%
bright
94%
ladder+
Bands are binned by measured luminance; confidence is endpoint call agreement.
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DNA Gel Electrophoresis
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Curio Design
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